Journal: Einstein

Article Title: Regulatory T cells isolated from endometriotic peritoneal fluid express a different number of Toll-like receptors

doi: 10.31744/einstein_journal/2020AO5294

Figure Lengend Snippet: Expression of Toll-like receptors by peritoneal regulatory T-cells from patients with endometriosis and controls

Article Snippet: Foram usados testes TaqMan de expressão gênica (Applied Biosystems) para os genes-alvo TLR1 (Hs00413978_m1), TLR2 (Hs00610101_m1), TLR3 (Hs01551078_m1), TLR4 (Hs00152939_m1), TLR5 (Hs00152825_m1), TLR6 (Hs00271977_s1), TLR7 (Hs00152971_m1), TLR8 (Hs00152972_m1) e TLR9 (Hs00152973_m1).

Techniques: Expressing, Control

Journal: Einstein

Article Title: Regulatory T cells isolated from endometriotic peritoneal fluid express a different number of Toll-like receptors

doi: 10.31744/einstein_journal/2020AO5294

Figure Lengend Snippet: Expressão de receptores do tipo Toll por linfócitos T reguladores peritoneais de pacientes com endometriose e controles

Article Snippet: Foram usados testes TaqMan de expressão gênica (Applied Biosystems) para os genes-alvo TLR1 (Hs00413978_m1), TLR2 (Hs00610101_m1), TLR3 (Hs01551078_m1), TLR4 (Hs00152939_m1), TLR5 (Hs00152825_m1), TLR6 (Hs00271977_s1), TLR7 (Hs00152971_m1), TLR8 (Hs00152972_m1) e TLR9 (Hs00152973_m1).

Techniques:

Journal: BMJ Open Diabetes Research & Care

Article Title: TLR5 influences the development of type 1 diabetes

doi: 10.1136/bmjdrc-2025-005111

Figure Lengend Snippet: Toll-like receptor 5 ( TLR5 ) gene expression. Boxes represent the 25% and 75% quartiles and whiskers indicate 1.5× the IQR. All significant p values and tendencies (p<0.1) are shown in the figure. The remaining group comparisons were non-significant. Taking all 12 autoantibody-positive persons together, they tended to be significantly higher than patients with newly diagnosed T1D (p=0.059). AB+, non-diabetic, autoimmune, single autoantibody-positive donors, network for Pancreatic Organ Donors with Diabetes (nPOD) (n=8); AB2+, non-diabetic, autoimmune, double autoantibody-positive donors, nPOD (n=4); CTR, non-diabetic controls (n=18); T1D (median 5 years), donors with intermediate-duration type 1 diabetes, nPOD (n=20); T1D (median disease duration, 35 days), donors with newly diagnosed type 1 diabetes, Diabetes Virus Detection study (DiViD) (n=5); T2D (median 2 years), donors with type 2 diabetes, nPOD (n=8).

Article Snippet: RNA isolation was performed as previously described. cDNA synthesis was performed using the High-Capacity cDNA Reverse Transcriptase Kit (Applied Biosystems, Foster City, California, USA), followed by quantitative PCR (qPCR) analysis of Tlr5 and Cd3 (Probe IDs: TLR5, Mm00546288_s1; Cd3, Mm01179194_m1; Applied Biosystems) using TaqMan gene expression assays on a Bio-Rad C1000 Touch CFX96 Real-Time System Thermal Cycler (Bio-Rad, Hercules, California, USA), as previously described.

Techniques: Gene Expression, Virus

Journal: BMJ Open Diabetes Research & Care

Article Title: TLR5 influences the development of type 1 diabetes

doi: 10.1136/bmjdrc-2025-005111

Figure Lengend Snippet: Association between islet TLR5 expression and T lymphocyte numbers. Correlation of islet TLR5 gene expression in donors with newly diagnosed type 1 diabetes, Diabetes Virus Detection study DiViD (n=5), and intraislet CD3+ cells. Each islet is normalized to the area of an IEQ, defined as an islet with a diameter of 150 µm or an average cross-sectional area of 0.010936 mm 2 . TLR5, Toll-like receptor 5.

Article Snippet: RNA isolation was performed as previously described. cDNA synthesis was performed using the High-Capacity cDNA Reverse Transcriptase Kit (Applied Biosystems, Foster City, California, USA), followed by quantitative PCR (qPCR) analysis of Tlr5 and Cd3 (Probe IDs: TLR5, Mm00546288_s1; Cd3, Mm01179194_m1; Applied Biosystems) using TaqMan gene expression assays on a Bio-Rad C1000 Touch CFX96 Real-Time System Thermal Cycler (Bio-Rad, Hercules, California, USA), as previously described.

Techniques: Expressing, Gene Expression, Virus

Journal: BMJ Open Diabetes Research & Care

Article Title: TLR5 influences the development of type 1 diabetes

doi: 10.1136/bmjdrc-2025-005111

Figure Lengend Snippet: Association between pancreatic Tlr5 and Cd3 gene expression in diabetic NOD mice. Correlation of pancreatic Tlr5 and Cd3 gene expression in female NOD mice (n=10) diagnosed with diabetes by blood glucose levels >12 mmol/L. ΔCt values, that is, Ct gene−Ct reference gene, are shown and correlated by Spearman analysis. ΔCt, delta cycle threshold; NOD, non-obese diabetic.

Article Snippet: RNA isolation was performed as previously described. cDNA synthesis was performed using the High-Capacity cDNA Reverse Transcriptase Kit (Applied Biosystems, Foster City, California, USA), followed by quantitative PCR (qPCR) analysis of Tlr5 and Cd3 (Probe IDs: TLR5, Mm00546288_s1; Cd3, Mm01179194_m1; Applied Biosystems) using TaqMan gene expression assays on a Bio-Rad C1000 Touch CFX96 Real-Time System Thermal Cycler (Bio-Rad, Hercules, California, USA), as previously described.

Techniques: Gene Expression

Journal: PLoS ONE

Article Title: Genetic Variants in Toll-Like Receptors Are Not Associated with Rheumatoid Arthritis Susceptibility or Anti-Tumour Necrosis Factor Treatment Outcome

doi: 10.1371/journal.pone.0014326

Figure Lengend Snippet: Results of the case control association analysis in the Dutch discovery set.

Article Snippet: Replication studies were performed using TaqMan® SNP genotyping assays for SNPs rs2072493 in TLR5 (assay ID C__22273027_10) and rs3853839 in TLR7 (assay ID C___2259573_10).

Techniques: Control

Journal: PLoS ONE

Article Title: Genetic Variants in Toll-Like Receptors Are Not Associated with Rheumatoid Arthritis Susceptibility or Anti-Tumour Necrosis Factor Treatment Outcome

doi: 10.1371/journal.pone.0014326

Figure Lengend Snippet: Meta-analysis of case control studies for TLR5 rs2072493 and TLR7 rs3853839.

Article Snippet: Replication studies were performed using TaqMan® SNP genotyping assays for SNPs rs2072493 in TLR5 (assay ID C__22273027_10) and rs3853839 in TLR7 (assay ID C___2259573_10).

Techniques: Control

Journal: PLoS ONE

Article Title: Genetic Variants in Toll-Like Receptors Are Not Associated with Rheumatoid Arthritis Susceptibility or Anti-Tumour Necrosis Factor Treatment Outcome

doi: 10.1371/journal.pone.0014326

Figure Lengend Snippet: Results of the association analysis for anti-TNF treatment outcome after three months of treatment initiation.

Article Snippet: Replication studies were performed using TaqMan® SNP genotyping assays for SNPs rs2072493 in TLR5 (assay ID C__22273027_10) and rs3853839 in TLR7 (assay ID C___2259573_10).

Techniques:

Journal: Radiation Oncology Journal

Article Title: Natural radioprotectors and their impact on cancer drug discovery

doi: 10.3857/roj.2018.00381

Figure Lengend Snippet: Present status of the synthetic radioprotector/radiosensitizer compounds under clinical development

Article Snippet: CBLB502 (protectans; toll-like receptor 5 agonist) , Acute radiation syndrome [ ] , Under phase I trials (Cleveland BioLabs Inc., Buffalo, NY, USA).

Techniques: